Czech J. Anim. Sci., 2015, 60(8):375-382 | DOI: 10.17221/8407-CJAS

Apparent ileal digestibility of protein and amino acids in protein feedstuffs and trypsin activity in the small intestine in broiler chickensOriginal Paper

M. Foltyn, M. Lichovníková, V. Rada, A. Musilová
Department of Animal Breeding, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic

Coefficients of ileal apparent digestibility (CIAD) for crude protein (CP) and amino acids in five protein feedstuffs: corn distillers dried grains with solubles (DDGS), raw full-fat soybean (RFFSB), extruded full fat soybean (EFFSB), soybean meal (SBM), and rapeseed meal (RSM) were determined using 5-week-old male broiler chickens. Trypsin activity in digesta in the ileum and jejunum were also measured. CIAD of CP was the highest in SBM (0.73). The lowest (P < 0.05) CIAD of CP was determined for RFFSB (0.45). There was no significant difference in CIAD of CP between RSM (0.61) and DDGS (0.62). The CIAD values for all essential amino acids except Lys (0.52) were the lowest in RFFSB (P < 0.05). The CIAD of Lys was the lowest in DDGS (0.39, P < 0.05). The CIAD for all essential amino acids was the highest in SBM (P < 0.05). There was no significant difference between EFFSB and SBM (P > 0.05) in CIAD of Leu (0.71, 0.73), His (0.66, 0.69), Lys (0.70, 0.77), Met (0.74, 0.80), Thr (0.60, 0.62), and Val (0.65, 0.67). Both the feedstuff and the intestinal region and their interaction had a significant (P < 0.01) effect on trypsin activity. The CIAD of CP and amino acids were very high and comparable both in EFFSB and SBM. The CIAD values for CP and amino acids in DDGS and RSM were lower than in SBM, but they seemed to be comparable to each other.

Keywords: soybean meal; rapeseed meal; raw full fat soybean; extruded full fat soybean; DDGS

Published: August 31, 2015  Show citation

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Foltyn M, Lichovníková M, Rada V, Musilová A. Apparent ileal digestibility of protein and amino acids in protein feedstuffs and trypsin activity in the small intestine in broiler chickens. Czech J. Anim. Sci.. 2015;60(8):375-382. doi: 10.17221/8407-CJAS.
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    References

    1. Adedokun S.A., Utterback P., Parsons C.M., Adeola O., Lilburn M.S., Applegate T.J. (2009): Comparison of amino acid digestibility of feed ingredients in broilers, laying hens and caecectomised roosters. British Poultry Science, 50, 350-358. Go to original source... Go to PubMed...
    2. Almirall M., Francesch M., Perez-Vendrell A.M., Brufau J., Esteve-Garcia E. (1995): The differences in intestinal viscosity produced by barley and 0-glucanase alter digesta enzyme activities and heal nutrient digestibilities more in broiler chicks than in cocks. Journal of Nutrition, 125, 947-955. Go to PubMed...
    3. Batal A.B., Dale N.M. (2006): True metabolizable energy and amino acid digestibility of distillers dried grains with solubles. Journal of Applied Poultry Research, 15, 89-93. Go to original source...
    4. Bedford M.R. (1996): The effect of enzymes on digestion. Journal of Applied Poultry Research, 5, 370-378. Go to original source...
    5. Bell J.M. (1984): Nutrients and toxicants in rapeseed meal: a review. Journal of Animal Science, 58, 996-1010. Go to original source... Go to PubMed...
    6. Bell J.M. (1993): Factors affecting the nutritional value of canola meal: a review. Canadian Journal of Animal Sciences, 73, 689-697. Go to original source...
    7. Clarke E., Wiseman J. (2005): Effects of variability in trypsin inhibitor content of soya bean meals on true and apparent ileal digestibility of amino acids and pancreas size in broiler chicks. Animal Feed Science and Technology, 121, 125-138. Go to original source...
    8. Clarke E., Wiseman J. (2007): Effects of extrusion conditions on trypsin inhibitor activity of full fat soybeans and subsequent effects on their nutritional value for young broilers. British Poultry Science, 48, 703-712. Go to original source... Go to PubMed...
    9. de Araujo J.A., Sakomura N.K., da Silva E.P., Dorigam J.D.P., Donato D.Z., da Silva J.H.V., Fernandes J.B.K. (2014): Response of pullets to digestible lysine intake. Czech Journal of Animal Science, 59, 208-218. Go to original source...
    10. de Coca-Sinova A., Valencia D.G., Jimenez-Moreno E., Lazaro R., Mateos G.G. (2008): Apparent ileal digestibility of energy, nitrogen, and amino acids of soybean meals of different origin in broilers. Poultry Science, 87, 2613-2623. Go to original source... Go to PubMed...
    11. Foltyn M., Rada V., Lichovnikova M., Safarik I., Lohnisky A., Hampel D. (2013): Effect of extruded full-fat soybeans on performance, amino acids digestibility, trypsin activity, and intestinal morphology in broilers. Czech Journal of Animal Science, 58, 470-478. Go to original source...
    12. Foltyn M., Lichovnikova M., Rada V., Musilova A. (2014): Apparent ileal amino acids digestibility of diets with graded levels of corn DDGS and determination of DDGS amino acids digestibility by difference and regression methods in broilers. Czech Journal of Animal Science, 59, 164-169. Go to original source...
    13. Gertler A., Nitsan Z. (1970): The effect of trypsin inhibitors on pancreatopeptidase E, trypsin, chymotrypsin and amylase in the pancreas and intestinal tract of chicks receiving raw and heated soya-bean diets. British Journal of Nutrition, 24, 893-904. Go to original source... Go to PubMed...
    14. Gilani G.S., Cockell K.A., Sepehr E. (2005): Effects of antinutritional factors on protein digestibility and amino acid availability in foods. Journal of AOAC International, 88, 967-987. Go to original source... Go to PubMed...
    15. Han F.X., Ding A.L., Sun J.M., Li G.Y. (2005): Development of new soybean germplasm with null lipoxygenase and kunitz trypsin inhibitor genes. Acta Genetica Sinica, 32, 417-423. Go to PubMed...
    16. Huisman J. (1990): Antinutritional effects of legume seeds in piglets, rats and chickens. Ph.D. Thesis, Wageningen: University of Wageningen.
    17. Hymowitz T. (1986): Genetics and breeding of soybeans lacking the Kunitz trypsin inhibitor. In: Friedman M. (ed.): Nutritional and Toxicological Significance of Enzyme Inhibitors in Foods. Plenum Press, New York, USA, 291-298. Go to original source...
    18. Kadim I.T., Moughan P.J., Ravindran V. (2002): Ileal amino acid digestibility assay for the growing meat chicken - comparison of ileal and excreta amino acid digestibility in the chicken. British Poultry Science, 43, 588-597. Go to original source... Go to PubMed...
    19. Kong C., Adeola O. (2010): Apparent ileal digestibility of amino acids in feedstuffs for White Pekin ducks. Poultry Science, 89, 545-550. Go to original source... Go to PubMed...
    20. Kunitz M. (1947): Crystalline soybean trypsin inhibitor II. General properties. Journal of General Physiology, 30, 291-310. Go to original source... Go to PubMed...
    21. Lemme A., Ravindran V., Bryden W.L. (2004): Ileal digestibility of amino acids in feed ingredients for broilers. World's Poultry Science Journal, 60, 423-438. Go to original source...
    22. Makkink C.A., Negulescu G.P., Guixin Q., Verstegen M.W.A. (1994): Effect of dietary protein source on feed intake, growth, pancreatic enzyme activities and jejunal morphology in newly-weaned piglets. British Journal of Nutrition, 72, 353-368. Go to original source... Go to PubMed...
    23. Niederau C., Grendell J.H., Rothman S.S. (1986): Digestive end products release pancreatic enzymes from particulate cellular pools, particularly zymogen granules. Biochimica et Biophysica Acta (BBA) - General Subjects, 881, 281-291. Go to original source... Go to PubMed...
    24. Parsons C.M., Potter L., Brown R.D. (1982): Effects of dietary protein and intestinal microflora on excretion of amino acids in poultry. Poultry Science, 61, 939-946. Go to original source...
    25. Perttila S., Valaja J., Partanen K., Jalava T., Venalainen E. (2002): Apparent ileal digestibility of amino acids in protein feedstuffs and diet formulation based on total vs digestible lysine for poultry. Animal Feed Science and Technology, 98, 203-218. Go to original source...
    26. Ravindran V., Hew L.I., Ravindran G., Bryden W.L. (1999): A comparison of ileal digesta and excreta analysis for the determination of amino acid digestibility in food ingredients for poultry. British Poultry Science, 40, 266-274. Go to original source... Go to PubMed...
    27. Ravindran V., Hew L.I., Ravindran G., Bryden W.L. (2005): Apparent ileal digestibility of amino acids in dietary ingredients for broiler chickens. Animal Science, 81, 85-97. Go to original source...
    28. Rostagno H.S., Pupa J.M.R., Pack M. (1995): Diet formulation for broilers based on total versus digestible amino acids. Journal of Applied Poultry Research, 4, 293-299. Go to original source...
    29. Soares J.A., Stein H.H., Singh V., Shurson G.S., Pettigrew J.E. (2012): Amino acid digestibility of corn distillers dried grains with solubles, liquid condensed solubles, pulse dried thin stillage, and syrup balls fed to growing pigs. Journal of Animal Science, 90, 1255-1261. Go to original source... Go to PubMed...
    30. Valette P., Malouin H., Corring T., Savoie L., Gueugneau A.M., Berot S. (1992): Effects of diets containing casein and rapeseed on enzyme secretion from the exocrine pancreas in the pig. British Journal of Nutrition, 67, 215-222. Go to original source... Go to PubMed...

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